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人生长激素测定血清SPRI，纳米单层屋面和酶联免疫吸附测定比较分析
JoVE Journal
Biologia
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JoVE Journal Biologia
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
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人生长激素测定血清SPRI，纳米单层屋面和酶联免疫吸附测定比较分析

DOI:
10.3791/53508-v

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11:17 min

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January 07, 2016

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, , ,
1Department of Nanoscience,University of North Carolina at Greensboro, 2Center for Biotechnology, Genomics, and Health Research,University of North Carolina at Greensboro, 3HORIBA Scientific

Capítulos

  • 00:05Título
  • 01:15Prepare SPRi Chip for Antibody Array
  • 04:08SPRi Detection of Human Growth Hormone
  • 05:46ELISA Protocol
  • 08:21Results: SPRi, Nano-SPRi, and ELISA Analysis of Recombinant Human Growth Hormone
  • 09:53Conclusion

Summary

Tadução automática

Tadução automática

所提出的工作（SPRI）测定法，特别是用于在加标人血清重组人生长激素的检测，通过比较SPRI直接结果与市售的酶联免疫吸附试验评估直接和扩增表面等离子体共振成像的诊断潜力（ELISA）套件。

Tags

Keywords: SPRiNano-SPRiELISAHGHSerumBiomarker DetectionLabel-freeReal-time MonitoringBiochip Surface Chemistry11-mercaptoundecanoic Acid1-Ethyl-3-carbodiimideN-Hydroxysuccinimide

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