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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
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December 07, 2015
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Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.
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Nunes, P., Guido, D., Demaurex, N. Measuring Phagosome pH by Ratiometric Fluorescence Microscopy. J. Vis. Exp. (106), e53402, doi:10.3791/53402 (2015).
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