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En Endothelial Planar Cell Model for Imaging Immunologisk Synapse Dynamics
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Capítulos
- 00:05Título
- 01:06Endothelia and Lymphocyte Preparation
- 02:55Live-cell Imaging Set Up
- 05:37Th1 Effector/Memory-endothelial Cell Immunological Synapse (IS) Initiation
- 06:56Results: Representative Th1:Endothelial APC ISs
- 08:51Conclusion
Adaptive immunity is controlled by dynamic 'immunological synapses' formed between T cells and antigen presenting cells. This protocol describes methods for investigating endothelial cells both as understudied physiologic APCs and as a novel type of 'planar cellular APC model'.
