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마우스 비장에서 불변 자연 살해 T 세포를 분리하고 확장을위한 최적화 방법
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
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마우스 비장에서 불변 자연 살해 T 세포를 분리하고 확장을위한 최적화 방법

11,721 Views

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09:01 min

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October 29, 2015

DOI:

10.3791/53256-v

DOI:
10.3791/53256-v

•

09:01 min
October 29, 2015

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1 Views
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1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation,Ghent University Hospital, 2VIB Inflammation Research Center,Ghent University

Tags

Invariant Natural Killer T CellsINKT CellsMouse SpleenCell IsolationCell ExpansionCD1d TetramerFACSIn Vitro CultureRecombinant CytokinesTCR StimulationIL-7

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Govindarajan, S., Elewaut, D., Drennan, M. An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen. J. Vis. Exp. (104), e53256, doi:10.3791/53256 (2015).

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