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An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
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An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
Capítulos
- 00:05Título
- 01:06Enrichment, Detection and Purification of Mouse Invariant Natural Killer T (iNKT) Cells
- 04:55Mouse iNKT Cell Culture and Expansion
- 07:13Results: Absolute Number and Cytokine Producing Capacity of In Vitro Expanded iNKT Cells
- 08:30Conclusion
Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.
