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An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
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An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

11,765 Views

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09:01 min

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October 29, 2015

DOI:

10.3791/53256-v

DOI:
10.3791/53256-v

•

09:01 min
October 29, 2015

•

2 Views
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1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation,Ghent University Hospital, 2VIB Inflammation Research Center,Ghent University

Summary

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Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.

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Govindarajan, S., Elewaut, D., Drennan, M. An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen. J. Vis. Exp. (104), e53256, doi:10.3791/53256 (2015).

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