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JoVE Journal Neuroscience
Purification of Mouse Brain Vessels
 

Purification of Mouse Brain Vessels

Article DOI: 10.3791/53208-v • 07:32 min • November 10th, 2015
November 10th, 2015 •
1,2,3, 4, 4, 1,2,3
1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot

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We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.

Tags

Purification Mouse Brain Vessels Blood-brain Barrier Vascular System Molecules Immune Cells Brain Pathologies Glioblastoma Stroke Edema Epilepsy Degenerative Diseases Parkinson's Disease Alzheimer's Disease Brain Tumors Inflammatory Conditions Multiple Sclerosis Meningitis Sepsis-induced Brain Dysfunctions Cerebrovascular Physiology Primary Culture Cell Sorting Dissociated Brain Tissue Cell Polarity Morphology Intercellular Relationships Brain Vessel Fragments Endothelial Cells Tight Junctions Basal Lamina Pericytes Smooth Muscle Cells Perivascular Astrocyte Endfeet Membranes
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