/
/
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
9,079 Views
•
08:51 min
•
June 25, 2015
DOI:
Summary
Automatically generated
The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.
Read Article
Cite this Article
Berens, C., Bisle, S., Klingenbeck, L., Lührmann, A. Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling. J. Vis. Exp. (100), e52903, doi:10.3791/52903 (2015).
Copy