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Capítulos
- 00:05Título
- 01:35Making the Probe
- 04:02In Situ Hybridization
- 06:53Applying an Antibody and Counter Stain
- 08:56Results: Comparing Eubacterial and P. gingivalis-specific Probes and Using the Eubacterial Probe for General Bacterial Detection Purposes
- 10:32Conclusion
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
Tags
Keywords:
In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases
