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JoVE Journal Developmental Biology
Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
 

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Article DOI: 10.3791/52768-v • 07:07 min • July 10th, 2015
July 10th, 2015 •
1,2, 1,3, 1, 1, 3, 3, 3
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, 3Department of Molecular and Cellular Sports Medicine, German Sports University Cologne

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Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.

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Toxic Chemicals Endothelial Cell Migration Alkylating Chemical Warfare Agents Wound Healing Disorder Granulation Tissue Capillary Blood Vessels Vasculogenesis Angiogenesis Directed Migration Early Endothelial Cell Migration Pathophysiology Therapeutic Intervention Impaired Wound Healing Alkylating Agent Exposure Candidate Compounds Boyden Chamber Chemokinesis Wound Healing Assay Track Analysis Fluorescent Dye TMRM Mitochondrial Membrane Potential
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