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简化人力中性粒细胞胞外陷阱（英语教师）分离和处理

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JoVE Journal Imunologia e Infecção

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

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简化人力中性粒细胞胞外陷阱（英语教师）分离和处理

DOI:

10.3791/52687-v

•

09:43 min

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April 16, 2015

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Sara Najmeh*¹, Jonathan Cools-Lartigue*¹, Betty Giannias, Jonathan Spicer, Lorenzo E. Ferri

¹LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Capítulos

00:05Título
01:14Neutrophil Isolation from Whole Blood
03:38Neutrophil Extracellular Trap (NET) Generation and Isolation
05:24NET-cancer Cell Static Adhesion Assay
08:18Results: Simple Generation and Isolation of NETs from Human Whole Blood
09:12Conclusion

Summary

Tadução automática

在下面的协议中，我们描述了一个非常简单的方法来分离中性粒细胞外使用容易获得的试剂从人全血中的陷阱（母语）。然后，我们说明如何分离的母语可以与癌细胞在体外粘附试验中使用。

Tags

Keywords: Neutrophil Extracellular Traps (NETs)NET Isolation NET Handling Cell-free NET Isolation NET-based Assays NET Adhesion Assay A549 Lung Cancer Cells PMA Stimulation Density Gradient Separation DNase1

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