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Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
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Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
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March 26, 2015
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Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.
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CardiomyocyteHeart DevelopmentImmunofluorescenceEmbryonic Mouse HeartSarcomeresMyofibrilsIntercalated DiscsCostameresMyocardial DevelopmentStructural And Functional UnitsDevelopmental Heart DefectsCultured CardiomyocytesIn Vivo Mechanical InputsEndocardial CuesSensitivityResolutionCo-immunostainingFluorescent ProteinStructural ChangesMutationsDevelopmental Heart Defects
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Wilsbacher, L. D., Coughlin, S. R. Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart. J. Vis. Exp. (97), e52644, doi:10.3791/52644 (2015).
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