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Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
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Neurociência
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JoVE Journal Neurociência
Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

8,386 Views

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10:32 min

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April 15, 2015

DOI:

10.3791/52572-v

DOI:
10.3791/52572-v

•

10:32 min
April 15, 2015

•

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1Department of Neurobiology, George S. Wise Faculty of Life Sciences,Tel-Aviv University, 2Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia, 3School of Electrical Engineering,Tel-Aviv University, 4School of Physics and Astronomy,Tel-Aviv University, 5Department of Informatics, Bioengineering, Robotics and System Engineering,University of Genova

Summary

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This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.

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Modular Neuronal NetworksIn VitroNeuronal CircuitsNetwork PatterningSurface TreatmentCellular PlatingCulturingFunctional InterconnectivityMicromachined StructuresBiocompatible PolymersElectrophysiologyCalcium Imaging

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Cite this Article

Kanner, S., Bisio, M., Cohen, G., Goldin, M., Tedesco, M., Hanein, Y., Ben-Jacob, E., Barzilai, A., Chiappalone, M., Bonifazi, P. Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits. J. Vis. Exp. (98), e52572, doi:10.3791/52572 (2015).

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