Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
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April 15, 2015
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This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.
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Kanner, S., Bisio, M., Cohen, G., Goldin, M., Tedesco, M., Hanein, Y., Ben-Jacob, E., Barzilai, A., Chiappalone, M., Bonifazi, P. Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits. J. Vis. Exp. (98), e52572, doi:10.3791/52572 (2015).
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