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The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture
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The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture
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April 28, 2015
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Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.
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Materne, E., Maschmeyer, I., Lorenz, A. K., Horland, R., Schimek, K. M. S., Busek, M., Sonntag, F., Lauster, R., Marx, U. The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture. J. Vis. Exp. (98), e52526, doi:10.3791/52526 (2015).
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