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The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture

The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture

28,070 Views

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10:05 min

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April 28, 2015

DOI:

10.3791/52526-v

DOI:
10.3791/52526-v

•

10:05 min
April 28, 2015

•

22 Views
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1Medical Biotechnology,Technische Universität Berlin, 2TissUse GmbH, 3Fraunhofer IWS

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Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.

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Materne, E., Maschmeyer, I., Lorenz, A. K., Horland, R., Schimek, K. M. S., Busek, M., Sonntag, F., Lauster, R., Marx, U. The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture. J. Vis. Exp. (98), e52526, doi:10.3791/52526 (2015).

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