É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
배아 마우스의 뇌에 추적 조건부 유전 Transsynaptic
Capítulos
- 00:05Título
- 01:29Preparing and Fixing Tissues
- 03:04Freezing and Cryosectioning
- 04:26Tyramide Signal Amplification (TSA) for Tracer Visualization
- 08:14Development of the Neural Circuits Controlling the Reproductive Axis
- 10:06Conclusion
Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.
