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Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

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11:03 min

December 22, 2014

DOI:

11:03 min
December 22, 2014

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Summary

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Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.

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