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Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans

Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans

7,903 Views

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10:55 min

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February 22, 2015

DOI:

10.3791/52445-v

DOI:
10.3791/52445-v

•

10:55 min
February 22, 2015

•

6 Views
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1Institute for Society and Genetics,University of California, Los Angeles, 2Environmental Health Sciences,University of California, Los Angeles, 3California Nanosystems Institute, Department of Molecular and Medical Pharmacology,University of California, Los Angeles

Summary

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We describe the detailed steps of a high-throughput chemical assay in the nematode Caenorhabditis elegans used to assess germline toxicity. In this assay, disruption of germline function following chemical exposure is monitored using a fluorescent reporter specific to aneuploid embryos.

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Cite this Article

Parodi, D. A., Damoiseaux, R., Allard, P. Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans. J. Vis. Exp. (96), e52445, doi:10.3791/52445 (2015).

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