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神経筋接合部：測定シナプスサイズ、断片化および共焦点蛍光顕微鏡を用いてシナプスタンパク質の密度の変化
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Neurociência
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JoVE Journal Neurociência
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
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神経筋接合部：測定シナプスサイズ、断片化および共焦点蛍光顕微鏡を用いてシナプスタンパク質の密度の変化

21,072 Views

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12:18 min

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December 26, 2014

DOI:

10.3791/52220-v

DOI:
10.3791/52220-v

•

12:18 min
December 26, 2014

•

10 Views
, , , , , ,
1Physiology and Bosch Institute,University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, 3Advanced Microscopy Facility, Bosch Institute,University of Sydney

Tags

Neuromuscular JunctionSynapseConfocal MicroscopyAcetylcholine ReceptorSynaptic VesicleSynaptic ProteinFRETNeurotransmissionMuscle ContractionStructural ChangesMouse Model

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Tse, N., Morsch, M., Ghazanfari, N., Cole, L., Visvanathan, A., Leamey, C., Phillips, W. D. The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy. J. Vis. Exp. (94), e52220, doi:10.3791/52220 (2014).

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