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Live-imaging of the Drosophila Pupal Eye
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January 12, 2015
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This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.
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Hellerman, M. B., Choe, R. H., Johnson, R. I. Live-imaging of the Drosophila Pupal Eye. J. Vis. Exp. (95), e52120, doi:10.3791/52120 (2015).
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