In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.
Ankri, L., Yarom, Y., Uusisaari, M. Y. Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature. J. Vis. Exp. (92), e52068, doi:10.3791/52068 (2014).