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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

24,687 Views

08:46 min

October 30, 2014

DOI:

08:46 min
October 30, 2014

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Summary

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In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.

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