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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
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08:46 min
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October 30, 2014
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In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.
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Ankri, L., Yarom, Y., Uusisaari, M. Y. Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature. J. Vis. Exp. (92), e52068, doi:10.3791/52068 (2014).
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