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A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
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November 08, 2014
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The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount in situ hybridization (WISH) read-out.
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Poureetezadi, S. J., Donahue, E. K., Wingert, R. A. A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos. J. Vis. Exp. (93), e52063, doi:10.3791/52063 (2014).
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