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Chapitres
- 00:05Titre
- 01:33Preparation of Zebrafish Mating Chambers and Collection of Embryos
- 02:41Arraying and Chemically Treating Zebrafish Embryos
- 04:34Embryo Fixation and Chemical Screening
- 05:52Results: Zebrafish Embryonic High-throughput Chemical Screen
- 07:17Conclusion
The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount in situ hybridization (WISH) read-out.
