Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Caroline Bonnans; Marja Lohela; Zena Werb

doi:10.3791/51916

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Echtzeit-Imaging von myeloischen Zellen in Dynamics Apc Min / +</sup Darmtumoren durch Spinning-Disk-konfokale Mikroskopie

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Real-time Imaging of Myeloid Cells Dynamics in Apc^Min/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Please note that all translations are automatically generated. Click here for the English version.

Echtzeit-Imaging von myeloischen Zellen in Dynamics Apc Min / +</sup Darmtumoren durch Spinning-Disk-konfokale Mikroskopie

9,508 Views

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05:21 min

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October 06, 2014

DOI:

10.3791/51916-v

DOI:

10.3791/51916-v

•

05:21 min

October 06, 2014

•

8 Views

Caroline Bonnans*^1,2, Marja Lohela*², Zena Werb²

¹Department of Oncology,INSERM U661, Functional Genomic Institute, ²Department of Anatomy,University of California

Summary

By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the Apc^Min/+colorectal cancer model.

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Real-time Imaging Myeloid Cells Intestinal Tumors Spinning Disk Confocal Microscopy ApcMin/+ Mice C-fms-EGFP ACTB-ECFP Intravital Imaging Tumor Microenvironment Intestinal Adenomas Blood Vessels Neutrophils

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Echtzeit-Imaging von myeloischen Zellen in Dynamics<em> Apc<sup> Min / +</sup</em> Darmtumoren durch Spinning-Disk-konfokale Mikroskopie

Summary

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