Real-time Imaging of Myeloid Cells Dynamics in <em>Apc<sup>Min/+</sup></em> Intestinal Tumors by Spinning Disk Confocal Microscopy

Caroline Bonnans; Marja Lohela; Zena Werb

doi:10.3791/51916

Journal

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Imunologia e Infecção

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Real-time Imaging of Myeloid Cells Dynamics in Apc^Min/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Real-time Imaging of Myeloid Cells Dynamics in Apc^Min/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Real-time Imaging of Myeloid Cells Dynamics in Apc^Min/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

9,519 Views

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05:21 min

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October 06, 2014

DOI:

10.3791/51916-v

DOI:

10.3791/51916-v

•

05:21 min

October 06, 2014

•

8 Views

Caroline Bonnans*^1,2, Marja Lohela*², Zena Werb²

¹Department of Oncology,INSERM U661, Functional Genomic Institute, ²Department of Anatomy,University of California

Summary

By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the Apc^Min/+colorectal cancer model.

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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Summary

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Real-time Imaging of Myeloid Cells Dynamics in Apc^Min/+ Intestinal Tumors by Spinning Disk Confocal Microscopy