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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

DOI:
10.3791/51916-v

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05:21 min

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October 06, 2014

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, ,
1Department of Oncology,INSERM U661, Functional Genomic Institute, 2Department of Anatomy,University of California

Chapters

  • 00:05Title
  • 00:59Preparation of the Intestine for Imaging
  • 02:43Image Acquisition
  • 03:36Results: Representative Spinning Disk Confocal Images of Non-tumoral and Tumoral Tissues from ApcMin/+ Mice
  • 04:57Conclusion

Summary

自动翻译

自动翻译

By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

Tags

Real-time ImagingMyeloid CellsIntestinal TumorsSpinning Disk Confocal MicroscopyApcMin/+ MiceC-fms-EGFPACTB-ECFPIntravital ImagingTumor MicroenvironmentIntestinal AdenomasBlood VesselsNeutrophils

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