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PNA-FISHによるマウスのBリンパ球における染色体異常の迅速分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
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PNA-FISHによるマウスのBリンパ球における染色体異常の迅速分析

16,744 Views

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07:54 min

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August 19, 2014

DOI:

10.3791/51806-v

DOI:
10.3791/51806-v

•

07:54 min
August 19, 2014

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3 Views
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1Department of Molecular Biology and Biochemistry,Rutgers, the State University of New Jersey

Summary

Automatically generated

DNA修復経路は、ゲノムの完全性の維持および変異および癌を予防するために必須である。このプロトコルの目的は、中期の染色体異常の直接観察によりゲノム不安定性を定量化することであるテロメアDNA反復のためのin situハイブリダイゼーション （FISH）、 蛍光を使用して、マウスのB細胞から広がる。

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Cite this Article

Misenko, S. M., Bunting, S. F. Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH. J. Vis. Exp. (90), e51806, doi:10.3791/51806 (2014).

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