このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
16,757 Views
•
07:54 min
•
August 19, 2014
DOI:
概要
Automatically generated
DNA repair pathways are essential for maintenance of genomic integrity and preventing mutation and cancer. The goal of this protocol is to quantify genomic instability by direct observation of chromosome aberrations in metaphase spreads from mouse B cells using fluorescent in situ hybridization (FISH) for telomeric DNA repeats.
Read Article
Cite this Article
Misenko, S. M., Bunting, S. F. Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH. J. Vis. Exp. (90), e51806, doi:10.3791/51806 (2014).
Copy