Journal
/
Neurociencias
/
Primary Culture of Mouse Dopaminergic Neurons
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Primary Culture of Mouse Dopaminergic Neurons

Primary Culture of Mouse Dopaminergic Neurons

36,757 Views

•

11:58 min

•

September 08, 2014

DOI:

10.3791/51751-v

DOI:
10.3791/51751-v

•

11:58 min
September 08, 2014

•

43 Views
, ,
1CNRS, UMR-5203,Institut de Génomique Fonctionnelle, Montpellier, 2Inserm,U661, Montpellier, 3Universités de Montpellier

Summary

Automatically generated

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

Explore More Videos

Primary CultureMouse Dopaminergic NeuronsParkinson’s DiseaseNigrostriatal NeuronsNeurodegenerationVentral MesencephalonTransgenic Mouse ModelsImmunofluorescenceQPCRSecond MessengersNeuronal Death/survival

Read Article

Cite this Article

Gaven, F., Marin, P., Claeysen, S. Primary Culture of Mouse Dopaminergic Neurons. J. Vis. Exp. (91), e51751, doi:10.3791/51751 (2014).

Download .ris file

Copy

Share Video

.

Copy