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Imunologia e Infecção
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Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

DOI:
10.3791/51747-v

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09:11 min

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September 25, 2014

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, ,
1Pneumococcal Research,Murdoch Childrens Research Institute, 2Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Capítulos

  • 00:05Título
  • 01:20Preparing the Latex Reagent
  • 04:21The Latex Agglutination Test with Pneumococcal Cultures
  • 06:55Scoring the Agglutination Test and Identifying the Serotype
  • 08:29Conclusion

Summary

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Latex agglutination testing is a simple, rapid and inexpensive method for serotyping Streptococcus pneumoniae, and has also been widely applied in diagnostic microbiology. This manuscript describes the in-house production of latex agglutination reagents, quality control procedures and the application of this technique to pneumococcal serotyping.

Tags

Streptococcus PneumoniaePneumococcusCapsular SerotypingLatex AgglutinationQuellung TestMicrobial DiagnosisIdentificationEpidemiologyVaccination ImpactResource-poor SettingsHigh-volume WorkloadsIn-house ProductionQuality Control

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