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Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

21,442 Views

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09:11 min

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September 25, 2014

DOI:

10.3791/51747-v

DOI:
10.3791/51747-v

•

09:11 min
September 25, 2014

•

17 Views
, ,
1Pneumococcal Research,Murdoch Childrens Research Institute, 2Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

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Latex agglutination testing is a simple, rapid and inexpensive method for serotyping Streptococcus pneumoniae, and has also been widely applied in diagnostic microbiology. This manuscript describes the in-house production of latex agglutination reagents, quality control procedures and the application of this technique to pneumococcal serotyping.

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Streptococcus PneumoniaePneumococcusCapsular SerotypingLatex AgglutinationQuellung TestMicrobial DiagnosisIdentificationEpidemiologyVaccination ImpactResource-poor SettingsHigh-volume WorkloadsIn-house ProductionQuality Control

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Cite this Article

Porter, B. D., Ortika, B. D., Satzke, C. Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination. J. Vis. Exp. (91), e51747, doi:10.3791/51747 (2014).

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