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Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces
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JoVE Journal Química
Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

Stabilizing Hepatocellular Phenotype Using Optimized Synthetic Surfaces

DOI:
10.3791/51723-v

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08:50 min

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September 26, 2014

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, , , , , , , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh, 2School of Chemistry,University of Edinburgh, 3MRC Centre for Inflammation Research,University of Edinburgh

Capítulos

  • 00:05Título
  • 01:37Synthesis of PHNGAD (Poly[1,6-hexanodiol/neopentyl glycol/di(ethylene glycol)-alt-adipic acid]diol
  • 02:53Synthesis of PU134
  • 04:18Preparation of PU134 Solutions and Coating of Glass Coverslips
  • 05:22Irradiation of Coverslip and Cell Culture and Differentiation
  • 06:09Results: Characterization of Polymer Coated Surfaces for Biological Applications
  • 08:17Conclusion

Summary

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This article will focus on developing polymer coated surfaces for long-term, stable culture of stem cell derived human hepatocytes.

Tags

HepatocytePrimary HepatocyteStem Cell-derived HepatocyteCell PhenotypeBiological MatrixSynthetic Polymer CoatingPolyurethaneCell CultureLong-term CulturePhenotype StabilityHigh-throughput ScreeningCellular Therapy

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