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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
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重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em

DOI:
10.3791/51583-v

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07:35 min

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June 09, 2014

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, ,
1Department of Biomedical Engineering,Duke University, 2Research Triangle MRSEC,Duke University

Capítulos

  • 00:05Título
  • 01:37Expression of Elastin-like Polypeptides (ELPs)
  • 02:22Purification of ELPs by Inverse Transition Cycling
  • 04:17Removal of Contaminants from ELP through Subsequent Rounds of ITC
  • 05:22Characterization of ELP Tt by Temperature-programmed Turbidimetry
  • 06:16Results: Characterization of ELPs Purified from E. coli through ITC
  • 07:13Conclusion

Summary

Tadução automática

Tadução automática

弹性蛋白样多肽是刺激 - 反应的生物聚合物与应用，从重组蛋白纯化给药。这个协议描述了来自大肠杆菌的使用其低临界溶解温度的相变行为作为一个简单的替代色谱弹性蛋白样多肽和其肽或蛋白质融合的纯化和表征。

Tags

Elastin-like PolypeptidesRecombinantNon-chromatographic PurificationLower Critical Solution TemperaturePhase TransitionCoacervationEscherichia ColiPeptide FusionProtein FusionBiosensingDrug DeliveryThermal Properties

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