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内吞体动力学在生活神经末梢与四维荧光成像可视化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

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内吞体动力学在生活神经末梢与四维荧光成像可视化

DOI:

10.3791/51477-v

•

10:51 min

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April 16, 2014

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Richard S. Stewart, Ilona M. Kiss, Robert S. Wilkinson

¹Department of Cell Biology and Physiology,Washington University School of Medicine

Capítulos

00:05Título
01:10Staining the Preparation with Supravital Dyes
02:30Configuring the Preparation for Imaging
03:31Setting Parameters for 4D Imaging
04:52Data Analysis
06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
10:23Conclusion

Summary

Tadução automática

四维（4D）成像被用于研究中两种类型的核内体的活的脊椎动物神经终端的行为和交互作用。这些小的结构的运动的特征是在三维空间中，从而允许确认如内涵体融合和胞吐作用的事件。

Tags

Keywords: 4D Fluorescence Imaging Endosome Dynamics Nerve Terminals Macroendosomes Acidic Endosomes 3D Time-lapse Deconvolution Quantitative Analysis

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