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RNAscope的<em>原位</em>检测转录活性的人类乳头状瘤病毒在头颈部鳞状细胞癌
JoVE Journal
Medicina
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JoVE Journal Medicina
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
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RNAscope的<em>原位</em>检测转录活性的人类乳头状瘤病毒在头颈部鳞状细胞癌

DOI:
10.3791/51426-v

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10:26 min

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March 11, 2014

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, , , , , , , , , ,
1Advanced Cell Diagnostics, Inc.

Capítulos

  • 00:05Título
  • 02:14Sample, Equipment, and Reagent Preparation
  • 04:07RNAscope Assay
  • 08:15Results: RNAscope Assay Detects HR-HPV in FFPE Tumor Tissue Sections
  • 09:45Conclusion

Summary

Tadução automática

Tadução automática

在头颈部肿瘤组织中高危型HPV的存在是有利的结果有关。 原位杂交技术最近开发的RNA称为RNAscope允许的HPV E6/E7 mRNA在FFPE组织切片直接可视化。

Tags

RNAscopeIn Situ DetectionTranscriptionally Active Human PapillomavirusHead And Neck Squamous Cell CarcinomaE6/E7 MRNAQRT-PCRHPV16HPV18HPV31HPV33HPV35HPV52HPV58Prognostic BiomarkerOropharyngeal Cancer

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