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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
JoVE Journal
Medicine
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JoVE Journal Medicine
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

DOI:
10.3791/51426-v

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10:26 min

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March 11, 2014

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, , , , , , , , , ,
1Advanced Cell Diagnostics, Inc.

Chapters

  • 00:05Title
  • 02:14Sample, Equipment, and Reagent Preparation
  • 04:07RNAscope Assay
  • 08:15Results: RNAscope Assay Detects HR-HPV in FFPE Tumor Tissue Sections
  • 09:45Conclusion

Summary

Automatic Translation

Automatic Translation

The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

Tags

RNAscopeIn Situ DetectionTranscriptionally Active Human PapillomavirusHead And Neck Squamous Cell CarcinomaE6/E7 MRNAQRT-PCRHPV16HPV18HPV31HPV33HPV35HPV52HPV58Prognostic BiomarkerOropharyngeal Cancer

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