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抗核抗体筛选使用Hep-2细胞

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Anti-Nuclear Antibody Screening Using HEp-2 Cells

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抗核抗体筛选使用Hep-2细胞

132,367 Views

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13:01 min

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June 23, 2014

DOI:

10.3791/51211-v

DOI:

10.3791/51211-v

•

13:01 min

June 23, 2014

•

39 Views

Carol Buchner¹, Cassandra Bryant², Anna Eslami³, Gabriella Lakos⁴

¹IFA Development Manager,INOVA Diagnostics, Inc., ²IFA Development,INOVA Diagnostics, Inc., ³Product Manager, Rheumatology,INOVA Diagnostics, Inc., ⁴Medical Director,INOVA Diagnostics, Inc.

Summary

间接免疫荧光（IIF）的测定法在传统上被用于抗核抗体（ANA）的人血清中的检测。这些抗体的存在可以在全身性自身免疫性风湿病（SARD）帮助诊断。该协议演示了如何有效地进行IIF技术，准确检测这些自身抗体。

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