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Rekombinante Expression von Funktions Hämagglutinin und Neuraminidase-Proteine ​​aus dem Roman H7N9 Influenza-Virus mit dem Baculovirus-Expressionssystem
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
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Rekombinante Expression von Funktions Hämagglutinin und Neuraminidase-Proteine ​​aus dem Roman H7N9 Influenza-Virus mit dem Baculovirus-Expressionssystem

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12:18 min

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November 06, 2013

DOI:

10.3791/51112-v

DOI:
10.3791/51112-v

•

12:18 min
November 06, 2013

•

12 Views
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai

Summary

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Hier beschreiben wir einen Weg, korrekt gefaltete und funktionelle Influenza-Virus-Oberflächenantigene von den neuen chinesischen H7N9-Virus in Insektenzellen abgeleitet auszudrücken. Die Technik lässt sich an beliebigen Ektodomänen viraler oder zellulärer Oberflächenproteine ​​exprimieren.

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Cite this Article

Margine, I., Palese, P., Krammer, F. Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System. J. Vis. Exp. (81), e51112, doi:10.3791/51112 (2013).

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