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Expressie van Functionele Recombinant Hemagglutinine en neuraminidase Eiwitten uit de roman H7N9 Influenza Virus Met de baculovirusexpressiesysteem
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
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Expressie van Functionele Recombinant Hemagglutinine en neuraminidase Eiwitten uit de roman H7N9 Influenza Virus Met de baculovirusexpressiesysteem

23,542 Views

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12:18 min

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November 06, 2013

DOI:

10.3791/51112-v

DOI:
10.3791/51112-v

•

12:18 min
November 06, 2013

•

12 Views
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai

Summary

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Hier beschrijven we een manier om correct gevouwen en functionele oppervlak influenzavirus-antigenen afgeleid van de nieuwe Chinese H7N9 virus in insectencellen uiten. De techniek kan worden aangepast aan ectodomeinen van elke virale of cellulaire oppervlakte-eiwitten tot expressie.

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Margine, I., Palese, P., Krammer, F. Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System. J. Vis. Exp. (81), e51112, doi:10.3791/51112 (2013).

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