Journal
/
Neurociência
/
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

DOI:
10.3791/51021-v

•

12:02 min

•

January 23, 2014

•

,
1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Capítulos

  • 00:05Título
  • 01:45Removal of Embryos and Dissection of Telencephalon
  • 02:53Cell Isolation
  • 04:43Magnetic Labeling and Separation
  • 07:26Cultured Periventricular Endothelial Cell Characterization
  • 11:24Conclusion

Summary

Tadução automática

Tadução automática

This video demonstrates an easy and reliable strategy for preparation of pure cultures of endothelial cells from the embryonic forebrain within 10-12 days and will be useful for research focused on many aspects of cerebral angiogenesis.

Tags

Embryonic ForebrainEndothelial CellsPial Vascular NetworkPeriventricular Vascular NetworkIsolationCulturePurificationCD31/PECAM-1AngiogenesisNeurovascular Development

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

Isolation and Culture of Embryonic Mouse Neural Stem Cells

Read Article