This video demonstrates an easy and reliable strategy for preparation of pure cultures of endothelial cells from the embryonic forebrain within 10-12 days and will be useful for research focused on many aspects of cerebral angiogenesis.
Kumar T., P., Vasudevan, A. Isolation and Culture of Endothelial Cells from the Embryonic Forebrain. J. Vis. Exp. (83), e51021, doi:10.3791/51021 (2014).