Journal
/
Neurociência
/
一个<em在体外</em从成年小鼠的肌间神经丛制备隔离肠道神经元和神经胶质
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
Please note that all translations are automatically generated. Click here for the English version.

一个<em在体外</em从成年小鼠的肌间神经丛制备隔离肠道神经元和神经胶质

26,101 Views

•

10:34 min

•

August 07, 2013

DOI:

10.3791/50688-v

DOI:
10.3791/50688-v

•

10:34 min
August 07, 2013

•

18 Views
, , , ,
1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Summary

Automatically generated

We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

Explore More Videos

Enteric Nervous SystemMyenteric PlexusIn Vitro PreparationIsolated NeuronsEnteric GliaMouse IleumColonEnzymatic DigestionNeurite OutgrowthAction PotentialsGFAP StainingImmunohistochemistryElectrophysiologyCalcium ImagingSingle-cell PCRGenetically Modified Animals

Read Article

Cite this Article

Smith, T. H., Ngwainmbi, J., Grider, J. R., Dewey, W. L., Akbarali, H. I. An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse. J. Vis. Exp. (78), e50688, doi:10.3791/50688 (2013).

Download .ris file

Copy

Share Video

.

Copy