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Mesurer la croissance et Gene Expression Dynamique de tumeur ciblée<em> S. Typhimurium</em> Bacteria
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1Health Sciences and Technology,Massachusetts Institute of Technology, 2Department of Bioengineering,University of California, San Diego , 3Biocircuits Institute,University of California, San Diego , 4Molecular Biology Section, Division of Biological Science,University of California, San Diego , 5Broad Institute of Harvard and MIT, 6Department of Medicine,Brigham and Women’s Hospital, 7Electrical Engineering and Computer Science and David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 8Howard Hughes Medical Institute
Capítulos
- 00:01Título
- 00:56Cell preparation and subcutaneous injection
- 01:34Subcutaneous injection of cancer cells
- 02:19Bacterial preparation for injection
- 03:01Tail vein injection of bacteria
- 03:44IVIS imaging of bacteria tumors
- 04:11Organ dissection
- 04:43Quantifying bacterial distribution in tumors
- 05:08Discussion and Representative Results
- 07:38Conclusions
Le but de ces expériences est de générer des données temps-parcours quantitatives sur la croissance et la dynamique de l'expression des gènes des atténué<em> S. typhimurium</em> Colonies bactériennes croissantes à l'intérieur des tumeurs. Cette vidéo couvre la préparation des cellules tumorales et l'implantation, la préparation de bactéries et d'injection, l'imagerie animal entier luminescence, excision de la tumeur, et le comptage de colonies bactériennes.
