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Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria
 

Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria

Article DOI: 10.3791/50540-v • 08:11 min • July 6th, 2013
July 6th, 2013 •
*1, *2, 2,3,4, 1,5,6,7,8
1Health Sciences and Technology, Massachusetts Institute of Technology, 2Department of Bioengineering, University of California, San Diego, 3Biocircuits Institute, University of California, San Diego, 4Molecular Biology Section, Division of Biological Science, University of California, San Diego, 5Broad Institute of Harvard and MIT, 6Department of Medicine, Brigham and Women's Hospital, 7Electrical Engineering and Computer Science and David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 8Howard Hughes Medical Institute
* These authors contributed equally

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Summary
Automatic Translation

The goal of these experiments is to generate quantitative time-course data on the growth and gene expression dynamics of attenuated S. typhimurium bacterial colonies growing inside tumors. This video covers tumor cell preparation and implantation, bacteria preparation and injection, whole-animal luminescence imaging, tumor excision, and bacterial colony counting.

Tags

Measuring Growth Gene Expression Dynamics Tumor-targeted S. Typhimurium Bacteria Quantitative Time-course Data Attenuated Strains Xenograft Tumors Human Ovarian Cancer Cell Line Luciferase Plasmid Visualization Non-virulent Bacteria Intravenous Injection Dosage Variation Tumor-localized Gene Expression Real-time Monitoring In Vivo Imaging System Excised Tumors Bacterial Colonies Quantitation
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