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长时程增强一个非常稳定的和可重复的记录和保存海马鼠标片制备工艺的改进
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Neurociência
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JoVE Journal Neurociência
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
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长时程增强一个非常稳定的和可重复的记录和保存海马鼠标片制备工艺的改进

DOI:
10.3791/50483-v

•

09:39 min

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June 26, 2013

•

,
1Department of Neurosciences, Research Institute for Biosciences,University of Mons

Capítulos

  • 00:05Título
  • 01:29Preparation of the Electrophysiological Rig and the Brain Slice Recording Chamber
  • 02:27Brain Removal and Hippocampal Dissection
  • 04:51Hippocampal Slices Preparation
  • 05:56Incubation of Slices in the Chamber
  • 07:02Results: Induction of LTP in Hippocampal Slices
  • 09:04Conclusion

Summary

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Tadução automática

本文提出了一种完整的方法来准备和保存<em>在体外</em>急性成年小鼠海马脑片。该协议允许非常稳定持久的长时程增强（LTP）的记录超过8小时，95％的成功率。

Tags

Hippocampal SlicesLong-term PotentiationLTPSynaptic PlasticityMemory EncodingCA1 RegionExperimental ConditionsOxygenationTemperatureHumiditySlice OrientationSlice ViabilityReproducibilityStable LTPMolecular MechanismsSynaptic Strength

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