Journal
/
Neurociência
/
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

DOI:
10.3791/50483-v

•

09:39 min

•

June 26, 2013

•

,
1Department of Neurosciences, Research Institute for Biosciences,University of Mons

Capítulos

  • 00:05Título
  • 01:29Preparation of the Electrophysiological Rig and the Brain Slice Recording Chamber
  • 02:27Brain Removal and Hippocampal Dissection
  • 04:51Hippocampal Slices Preparation
  • 05:56Incubation of Slices in the Chamber
  • 07:02Results: Induction of LTP in Hippocampal Slices
  • 09:04Conclusion

Summary

Tadução automática

Tadução automática

This paper presents a complete methodology to prepare and preserve in vitro acute hippocampal slices from adult mice. This protocol allows the recording of very stable long-lasting long-term potentiation (LTP) for more than 8 hours with a success rate of 95%.

Tags

Hippocampal SlicesLong-term PotentiationLTPSynaptic PlasticityMemory EncodingCA1 RegionExperimental ConditionsOxygenationTemperatureHumiditySlice OrientationSlice ViabilityReproducibilityStable LTPMolecular MechanismsSynaptic Strength

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Long-term Time Lapse Imaging of Mouse Cochlear Explants

A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Read Article