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Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
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Neuroscienze
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JoVE Journal Neuroscienze
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

DOI:
10.3791/50483-v

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09:39 min

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June 26, 2013

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,
1Department of Neurosciences, Research Institute for Biosciences,University of Mons

Capitoli

  • 00:05Titolo
  • 01:29Preparation of the Electrophysiological Rig and the Brain Slice Recording Chamber
  • 02:27Brain Removal and Hippocampal Dissection
  • 04:51Hippocampal Slices Preparation
  • 05:56Incubation of Slices in the Chamber
  • 07:02Results: Induction of LTP in Hippocampal Slices
  • 09:04Conclusion

Summary

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This paper presents a complete methodology to prepare and preserve in vitro acute hippocampal slices from adult mice. This protocol allows the recording of very stable long-lasting long-term potentiation (LTP) for more than 8 hours with a success rate of 95%.

Tags

Hippocampal SlicesLong-term PotentiationLTPSynaptic PlasticityMemory EncodingCA1 RegionExperimental ConditionsOxygenationTemperatureHumiditySlice OrientationSlice ViabilityReproducibilityStable LTPMolecular MechanismsSynaptic Strength

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